The purification unit consisted of a Sep-Pak C18 cartridge (Waters, 1 g C18). Purity. 3. Silica-based bonded phase with strong hydrophobicity; used to adsorb analytes of even weak hydrophobicity from aqueous solutions; typical applications . Alumina-N Plus Cartridges were treated with 10 mL water. . The 2.5 M NaOAc was prepared using sodium acetate trihydrate powder from Sigma-Aldrich (St. Louis, MO, USA). Laboratories (Muskegon, Michigan); Sep Pak C18 cartridges In this study, we evaluated two methods of column chro- and JU, Bondapak C18 (10 /urn) liquid chromatography col- matography for separation and purification of the A14 spe- umns for use in conjunction with a Waters HPLC were ob- cies of insulin. Load acidified digest prepared in Step 4c onto equilibrated Sep-Pak cartridge. ReproSil C18-AQ resin (3 m particle size) was purchased from .

Materials and ReagentsSep-pak C18 desalting cartridges and XBridge C18 5 m 4.6 250 mm columns were from Waters (Milford, MA). Peptide was eluted using Round-bottom flask (Sigma-Aldrich, cat. testing before the reaction mixture was transferred onto a purification unit consisting of a preconditioned 500 mg C18, 3 cm 3, Sep-Pak cartridge, catalog number WAT036815 (Waters, MA, USA). Collect the flow through. The treatment was stopped by processing the samples (0.3 ml) on Sep-Pak Plus C18 cartridge 55-105 m cartridges (Waters Corporation, Milford, MA, USA) containing 130 mg of octadecyl (C18) material, which is known to quickly remove the SD agents and stop the reaction . View all Strata C18-E* parts *Strata C18-E is a recommended alternative to: Waters Sep-Pak tC18, Agilent Bond Elut C18, Biotage IST ISOLUTE C18 (EC),UCT CleanScreen C18, and Supelco Discovery DSC-18. Trypsin (Sigma, St. Louis, MO). A precoated Silica Gel 60 TLC plate was obtained from Merck (Germany). From this time . Available for Sale. Each separation is different and not all steps may be required for your application. Sep-Pak C18 Cartridges. Chemical standards of -pinene, linalool, -humulene ( -caryophyllene), nerol, 2-phenylethyl alcohol (PEA), -caryophyllene, citronellol, geraniol, methyl eugenol, and eugenol were supplied from Sigma Aldrich (USA) while NaCl .

Radiochemical purity was determined by radio-TLC as described above. . Sep-Pak C18 Cartridges. The cartridge was subsequently washed with 5 mL of saline, followed by elution of the trapped tracer with 70% ethanol (2 mL) and dilution with 0.9% NaCl solution for injection (8 mL). A small reversed-phase C18 cartridge (Sep-Pak C18, Waters Associates, Inc.) is used for this purpose. The preparation with a label was purified using C18 Sep-Pak cartridge and various types of cation exchangers. The LC-Pak is designed to be connected to the outlet of ultrapure water equipment, in particular, the Q-POD dispenser of Milli-Q systems and is warranted to deliver at least 500 liters. Their detection was possible using wide-bore capillary gas chromatography with SPB-1 and HP-17 wide-bore capillary columns with satisfactory separation from impurities. 50 mM ammonium hydrogen carbonate. The Sep-Pak C18 sorbent is made using the most reproducible bonding technology available, utilizing monofunctional silane chemistry. After washing the column with 0.1% TFA the bound material was eluted with 0.1% TFA in 80% acetonitrile (ACN). The trapped product [18 F]PyMHO, ([18 F]6) was eluted from the Sep-Pak cartridge with 1.5-mL acetonitrile. Eluates were then dried in vacuo. Adjust to pH 8.4 with 10% (v/v) ammonia solution. Sep-Pak C18 (500 mg) cartridge from Sigma-Aldrich was used for precolumn separation. Simultaneous Analysis of Amino and Organic Acids in Extracts of Plant Leaves astert-Butyldimethylsilyl Derivatives by Capillary Gas Chromatography Sep-Pak C18 Chromatography Cartridge. PNGaseF digested sample . For the Sep-Pak C18 Classic Cartridge, the best way to operate the cartridge is by using a syringe (e.g., Injekt 10 mL Luer Solo, Braun). The mixture containing high mannose N-linked glycan, deglycosylated ribonuclease B and PNGase F was then loaded to the cartridge. Waters Sep-Pak (WAT054955) for peptide desalting. SEM SS18R 5-50 l or 6-60 g. For reverse-phase cartridges, such as C18 Sep-Pak, the cartridge should be activated based on the manufacturer's instructions, usually by passing an organic solvent (ethanol) and water through it. If the sample is a solid, it must be dissolved or extracted prior to loading. All solutions were prepared and stored in metal-free polypropylene centrifuge tubes (VWR International, LLC). Condition a C18 Sep-Pak (50 mg) column with methanol, 5% acetic acid, 1-propanol and 5% acetic acid in succession. : 34118-92-8. Sep-Pak C18 plus light cartridge, 130 mg Sorbent per cartridge, 55-105 M particle size (Waters; cat. Le migliori offerte per (2-Pack) ACQUE 700001436 o-ring per 10x10mm della cartuccia della sono su eBay Confronta prezzi e caratteristiche di prodotti nuovi e usati Molti articoli con consegna gratis! Equilibrate a C18 cartridge with 3 mL of 100% acetonitrile, followed by 5 mL of 5% acetic acid. Isatin. The incuba-tion media and washings were similarly purified following acidification. Z723134) Sand-core funnel (National Institutes of Health (NIH) self-service store or institutional glassware shop) . It is conditioned with EtOH and water on the cassette through the synthesis program. To the collected HPLC fraction was added 10-mL water and passed through an activated Sep-Pak plus C18 cartridge, and the cartridge was washed with 5 mL of water. Thermo Scientific HyperSep C18 Cartridges feature a highly retentive alkyl-bonded phase for nonpolar to moderately polar compounds. 7 . Purification of radiopharmaceuticals is mostly performed using disposable cartridges or by HPLC. Waters Sep-Pak (WAT054955) for peptide desalting. Sigma Aldrich SigmaPrep Spin Columns SC1000-1KT. SigmaAldrich: T6567: C18 SepPak Cartridges: Waters: WAT054955: TMT10plex isobaric label reagent set: Thermo Fischer Scientific: 90406: TMT11131C Label Reagent: Sep-Pak Product Type Solid Phase Extraction Units per Package 100 pk Chemistry tC18 Endcapped Yes Format Vac Cartridge Mass Spec Compatibility Yes Mode Reversed Phase Particle Size 37-55 m Pore Size 125 Sorbent Substrate Silica Sorbent Weight 100 mg Water Wettable No pH Range Min 2 pH pH Range Max 8 pH Barrel Size 1 cc Hold up volume 0.25 mL Dried glycopeptides were resuspended in 25 mM sodium phosphate buffer, pH 7.5, for digestion . Sigma-Aldrich: 213462-25G: Methyl iodine: Sigma-Aldrich: 289566-100G: Methanol, anhydrous: Sigma-Aldrich: . Lab grade pyruvic acid used for the synthesis of pyranoanthocyanins was purchased from Sigma Aldrich (St. Louis, Missouri, U.S.). Sep-Pak C18 Vac cartridges contain a hydrophobic, reverse-phase, silica-based bonded phase that is used to adsorb analytes of even weak hydrophobicity from aqueous solutions. Solvolysis was performed at 50C for 1 hour using 5 ml of tetrahydrofuran containing 5 l of 4 M sulfuric acid. Saline solution (0.9% w/v) was purchased from Braun Medical Industries. Eluates were then dried in vacuo. United States Globally.

71196) at UM (note: 1.5 . urine, total bile acids were extracted on a Sep-Pak C18 cartridge. Prior to use, they were activated with 2 ml of methanol and 5 ml of water. Socios de primera lnea Merck Millipore + Sigma-Aldrich = la divisin Life Science de Merck que opera en los Estados Unidos y Canad como MilliporeSigma. Sep-Pak light (46 mg) Accell plus QMA carbonate cartridges and Sep-Pak C18 light cartridges were purchased from Waters Corporation. The stock solutions of vitamin B 1, B 2, B 3, B 5, B 6, and B 9 were prepared by dissolving 0.01 g of each standard in 50 ml of 0.1 m HCl in a 100 ml volumetric flask and diluted to the mark. Sep-Pak C18 Vac cartridges contain a hydrophobic, reverse-phase, silica-based bonded phase that is used to adsorb analytes of even weak hydrophobicity from aqueous solutions. # WAT023501) Reagents. Sep-Pak Classic C18 cartridges were purchased from Waters. Sep~Pak procedure The C18 Sep-Pak cartridges were attached to a Luer-tipped glass syringe. Triethylammonium acetate (TEAA) buffer was purchased from Sigma-Fluka. N-Acetylprocainamide hydrochloride. Samples were placed on the Sep-Pak cartridge, washed through with 10 ml TEAA, eluted with 10 ml 50% (v/v) aqueous methanol, reduced to low volume Journal of Plant Pathology (2009), 91 (1), 141-146 Both samples (solvolyzed and unsolvolyzed) were then a Sep-Pak C18 cartridge column clean up. Applied Filters: Keyword:'34118-92-8' Showing 1-1 of 1 result for " 34118-92-8 " within Products. The solution was withdrawn and passed through a Sep-Pak C18 Plus cartridge to remove the mobile phase. (Sigma, St. Louis, MO) in the dark for 30 min. Sort by Relevance. The sample application, washing and elution were carried out at a flow-rate not exceeding 10 ml/min. After washing the column with 0.1% TFA the bound material was eluted with 0.1% TFA in 80% acetonitrile (ACN). ton X-100 were purchased from Sigma. Trypsin buffer. Sigma-Aldrich (1) Formula Weight. A Sep-Pak C 18 cartridge was cleaned and solvated by washing three times with methanol (10 mL portions) and chloroform-methanol (2:1, 20 mL portions), methanol (10 mL) again and finally with 'Folch upper phase', i.e. Finally, metal ions may also originate from the cyclotron target, therefore the [ 18 F]F was trapped on Sep-Pak light Accel plus anion exchange cartridge and washed with HPCE grade water before it was used for radiolabeling. The reaction mixture was passed through the column after radiolabeling. Applied Filters: Keyword:'91-56-5' Showing 1-1 of 1 result for " 91-56-5 " within Products.

. The reactionvial and cartridge were washed with H 2O (5 mL), and the product was eluted with 200 mL of a 50% v/v EtOH/saline solution followed by 800 mL of normal saline (0.9% NaCl in deionized H 2O). . Samples containing plasma only, HCV-spiked plasma, or HCV-spiked plasma . Radiat. Sigma-Aldrich (1) Formula Weight. Markush Class. . The reduced peptide was further purified by RP-HPLC on Vydac C18 column (218TP510, Waters) equilibrated with 0.1% trifluoroacetic acid. pH was adjusted by addition of acetic acid 99.99%, Sigma-Aldrich). Sort by Relevance. High-performance liquid chromatography (HPLC) grade water and gold label acetonitrile were purchased from Fisher Scientific. The Sep-Pak C18 cartridge is then washed with 10 mL of equilibrium solvent. All conditioned cartridges were kept wet unless otherwise specified. The cartridges were of Sep Pac . 20cc, 1g LP 20/PK 328.00 SKU: WT186000117 Draw up 5 mL into the . The substrate .

CAS No. Purity. matrix active group C18-SD, volume 6 mL, pk of 30 NACRES: NB.21 Pricing and availability is not currently available. Purified human ZP were digested using trypsin (Sigma) and purified by reverse-phase Sep-Pak C18 cartridge (Waters Corp) as described (28). Expand. The C18 Sep-Pak cartridge is installed onto the synthesis module. Article . A type Dilute the mixture with 15 mL of HEPES buffer (0.1 M, pH 2) and pass it through a Sep-Pak C18 Plus light cartridge in order to trap the product on the solid phase. Each. . Reagents were purchased from Sigma-Aldrich (Milan, Italy) unless otherwise specified. Sep-Pak Plus Silica, Sep-Pak CM, Sep-Pak QMA, and Sep-Pak C18 cartridges, and bicinchoninic acid protein assay kit were purchased from Waters and Pierce, respec-tively. Reference article suggest that in sample preparation- after acid hydrolysis, the filtrates (0.50 ml) were applied to a Millipore SEP-PAK Clartridge (C18, 500 mg, Altech,Belgium), prewetted with. Retention was expressed as % of trapped 67 Ga on the silica cartridge from . Glycopeptides were eluted stepwise in 20% isopropyl alcohol in 5% AcOH and 40% isopropyl alcohol in 5% AcOH. C18 Plus and t C18 Cartridges were preconditioned with 1 mL ethanol, and followed by rinse with 10 mL water. The preparation with a label was purified using C18 Sep-Pak cartridge and various types of cation exchangers. Melting Point (C) Physical Form. (Sigma, cat. Both, the 20 and 40% isopropyl alcohol fractions were pooled and evaporated to dryness. Prepare sample: The sample can be applied to the Certified Sep-Pak cartridge in either liquid or a gas phase. 5 IP Buffer: 250 mM 3-(N-Morpholino)propanesulfonic acid (MOPS pH 7.2, 50 mM sodium phosphate, 250 mM sodium chloride. The benzodiazepine-containing samples, after heating in acid and centrifugation, were passed through the cartridges and eluted with chloroform. no. Placed at the point of dispense of Direct-Q, Milli-Q Direct / Reference, Simplicity and Synergy systems, Designed for trace and ultra-trace organic analysis by HPLC, UHPLC, LC-MS, and LC-MS/MS. Wash column sequentially with 1ml, 3mL, 5 mL .

The cartridge was rinsed with 8 ml 0.9 % . Research Area. Hide. Product Overview Videos Documents Recommendations Showing 10 of 14 Show more (4 mg/mL) diluted in 0.5 M H 2 SO 4 were loaded onto a conditioned Sep-Pak C18 cartridge (Waters, Milan, Italy), washed with 2.0 mL of H 2 SO 4 (5.0 mM) and eluted with 5.0 mL of methanol. 10 mm mL) and passed through a preactivated Sep-Pak C18 Light cartridge. SMM SS18R 50-150 l or 30-300 g. 3. the reaction mixture was purified using Sep-Pak plus C18 cartridge, and then the C18 cartridge was washed with 40 mL of water, finally, the C18 cartridge was . Free glycans are then eluted from the Carbograph cartridge with 3 c.v. of Solution A . Category: SamplePreparation&Filtration. DMT On Purification of DNA Oligonucleotides <35mer Using Oasis HLB SPE Products [Excerpt from Oasis Applications Notebook] 0.1 to 1.0 mol of DMT-on oligos can be cleaned up on Oasis HLB 6 cc/200 mg cartridges. a 10g C18 Sep-Pak cartridge (Waters, Milford, MA) equilibrated in 0.1% TFA and eluted with 40% acetonitrile. After application of the biological fluid, followed by an aqueous wash, estrogens are eluted . Below is a five step guideline for using Certified Sep-Pak cartridges. Available for Sale. The C18 SPE cartridge was sequentially rinsed with 10 mL of 100% ACN, 50% ACN, and water. . The dried sample is solubilized in 200 L of 0.1% (v/v) TFA and loaded directly onto the Sep-Pak C18 cartridge. The mixture was loaded onto a Sep-Pak C18 cartridge* (50 mg, 1 mL) that was pre-equilibrated with 0.1% trifluoroacetic acid (TFA). The PNGase F digested solution is boiled for 5min, passed through a 2g C18 Sep-Pak cartridge and washed with dH 2 O. Description SDS Pricing; 114618: 97% . Background: To optimize [18F] 9-fluoropropyl-(+)-dihydrotetrabenazine (18F-FP-(+)-DTBZ) purification via solid-phase extraction (SPE) with combined cartridges to facilitate its widespread clinical application.Methods: A modified SPE purification method, employing Sep-Pak PS-2 and Sep-Pak C18 cartridges, was used for the preparation of 18F-FP-(+)-DTBZ. Placed at the point of dispense of Milli-Q IQ/EQ water systems. The identity and purity of the product were confirmed by analytical HPLC. The radiochemical yields of the SiFA reaction become far more reliable when the strong anion exchange (SAX) cartridge (Sep-Pak Light (46 mg)) SPE-based Munich method (Step 26A) is used for 18 F . It features a silica-based octadecyl bonded phase with strong hydrophobicity which is used to adsorb analytes of even weak hydrophobicity from aqueous solutions.

methyl ester (Sigma), as an internal standard. Aliquots of these eluates were analyzed by re-verse-phase HPLC using a 0 to 100% methanol gradient a new radiotracer for clinical investigations in PET imaging of sigma(1) receptors in brain. All other reagents were of the highest purity available. The cartridges were of Sep Pac . chromatography C18 Cartridge Solid Phase Extraction (SPE) Waters Sep-Pak C18 (500 mg) was used. chloroform-methanol-water (3:48:47 by volume; 10 mL) containing 0.1M potassium chloride. Sep Pak Plus C18 cartridges (Waters, USA) and Isolute ENV+ (Biotage, USA) were used for solid phase extraction. The C18 was conditioned with 1 mL of absolute ethanol followed by 3 mL deionized water. . The eluate was lyophilized and resuspended in 0.1% acetic acid. SKU: WAT036945. The flow-through and 2 c.v. water wash are pooled and applied on a 1g Carbograph cartridge, which is subsequently washed with 6 c.v. dH 2 O. This method was compared to the . . The C18 solid phase extraction (SPE) cartridge uses octadecyl silica as a filler to retain non-polar compounds by strong hydrophobic interaction. After application of the biological fluid, followed by an aqueous wash, estrogens are eluted . Cell Culture C18 SepPak Cartridges: Waters: WAT054955: TMT10plex isobaric label reagent set: Thermo Fischer Scientific: 90406: TMT11131C Label Reagent: Thermo Fischer Scientific: . . The Sep-Pak tC18 contains more carbon and is therefore more hydrophobic than the C18. USP grade 3% hydrogen peroxide was manufactured by . QMA Carb Cartridges were treated with 10 mL 0.2 M K 2 CO 3 solution followed by 20 mL of water. VWR; VWRI470105-972 Sep-Pak C18 Chromatography Cartridge. The solution was loaded onto a Waters Sep- pak C18 cartridge for solid phase extraction (SPE). Stop the reaction by adding 2 drops of 5% acetic acid. Sep-Pak C18 Vac cartridge ,1 mL (100 mg sorbent) Waters: WAT023590: 2-mL screw cap microcentrifuge tube: USA Scientific: . a Sep-Pak C18 cartridge and eluted with methanol. Wash the column with 4 ml of 5% acetic acid and collect the wash fractions. To stop the digestion, 1% TFA was added, peptides were purified on Sep-Pak C18 Cartridge (Waters, Milford, MA) and either eluted in 80% acetonitrile (ACN) for high pH reverse phase chromatography or desalted on C18 StageTips (as described previously ). All other chemical reagents were purchased from Sigma-Aldrich or Fisher Scientific. Sep-Pak C18 SPE cartridges were used to separate the released N-linked glycans from the protein. The purified native N-glycans were permethylated as described (29), purified The N-glycans were then released by N-glycosidase F (Roche Applied Science) and purified on a Sep-Pak C18 cartridge. Sep-Pak C18 light cartridge . The reaction mixture was passed through the column after radiolabeling. SPE Cartridge, Chromafix C18 (S) 149.00 SKU: MN731801 In Stock Waters SPE Cartridge, Oasis HLB Cartridge 6cc,/500 mg, 30/PK 249.00 SKU: WT186000115 In Stock Waters SPE Cartridge, Oasis HLB HLB Cart 12cc,/500 mg, 60m 20/PK 219.00 SKU: WT186000116 In Stock Waters SPE Cartridge, Oasis HLB Extraktion Cart. After lyophilization, two-thirds of the radioactive product was digested overnight at 37 C with 0.6 units of -N-acetylglu-cosaminidase (NAG) from human placenta (Sigma) in 0.1M citrate/phosphate buffer, pH 5.5.

SEM SS18R 5-50 l or 6-60 g. The mixture was loaded onto a Sep-Pak C18 cartridge* (50 mg, 1 mL) that was pre-equilibrated with 0.1% trifluoroacetic acid (TFA). Sigma-Aldrich - Switzerland. Sep-Pak light QMA, Sep-Pak plus C18 were purchased from Waters Corporation (Milford, MA, USA). Waters has not evaluated Sep-Pak C18 for cleanup of DMT-off oligos. : 91-56-5. Product Description Sep-Pak C18 Vac cartridges contain a hydrophobic, reverse-phase, silica-based bonded phase that is used to adsorb analytes of even weak hydrophobicity from aqueous solutions. United States Globally. To ensure ultra-trace organic levels are reached, the LC-Pak technology is based on the proven C18 reverse-phase silica purification media. Properties Quality Level 100 material polypropylene filter (graded density pre-filter) feature endcapped packaging pk of 30 manufacturer/tradename CDS Analytical 98-0604-0199-3 extent of labeling 18% C loading bed I.D. 1-7. 1. Each. All other chemicals and reagents were purchased from Sigma-Aldrich, Fisher Scientific, and Tokyo Chemical Industry. Appl. . The filler retains most of the organic matter and is widely used in the fields of environmental and food safety. A simple extraction procedure with a Sep-Pak C18 cartridge for benzophenones and their detection after separation by wide-bore capillary gas chromatography (GC) are presented. Ovalbumin (1 mg, Sigma) was reduced, alkylated and digested overnight with trypsin in of 25 mM ammonium bicarbonate buffer (pH~8) at 37 C. Amberlite XAD-16 resin (Sigma-Aldrich) was used for separating phenolic acids from other compounds in wine and grape juice. Sep-Pak C18 3 cc Vac RC Cartridge, 500 mg Sorbent per Cartridge, 55-105 m, 50/pk. SigmaUltra Iodoacetamide (Sigma-Aldrich, I1149-5G) DL-Dithiothreitol (Sigma-Aldrich; cat. 2. A small reversed-phase C18 cartridge (Sep-Pak C18, Waters Associates, Inc.) is used for this purpose. SMM SS18R 50-150 l or 30-300 g. The upper phase from a Folch . (2 mm 10 mm) with sorbents such as Dowex 50W-X-12 (Fluka; Sigma-Aldrich), Purolite WCA 100 (Purolite, USA), KU-2 (Aquachem). Melting Point (C) Physical Form. The final sample solution of 1.5 mL 70% ethanol was loaded onto a 200 mg Certified Sep-Pak C18 cartridge pre-conditioned with 4 mL of absolute ethanol and with 6 mL of 70% ethanol. Sep-Pak Plus C18 cartridges (Waters, MA) were used for the purification of [64 Cu][Cu (ATSM)]. Compare Product No. 2.2 Chemical synthesis of H 2-ATSM precursor The column was then washed with acidified water (0.01% HCl) to . a Sep-Pak C18 cartridge previously activated with methanol, followed by 10 ml of 0.01 M triethylammoni-um acetate solution (TEAA). Samples were diluted with 50 mM ammonium bicarbonate, . Solvolyzed samples were neutralized with ethanolic 4 M NaOH prior to enzymatic hydrolysis. Markush Class. Local anaesthetics present in whole blood or cerebrospinal fluid (CSF) could be rapidly isolated by use of Sep-Pak C18 cartridges with chloroform/methanol as an elution solvent. (2 mm 10 mm) with sorbents such as Dowex 50W-X-12 (Fluka; Sigma-Aldrich), Purolite WCA 100 (Purolite, USA), KU-2 (Aquachem). At the first stage of wine sample preparation, the wine was concentrated from 100 to 90mL in . CAS No. Features of Reversed Phase C18 Solid Phase Extraction (SPE) Cartridges (11C)labeled ()methyl jasmonate was synthesized using a C18 Sep Pak at 100C to sustain a solidsupported 11Cmethylation reaction of sodium ()jasmonate using (11C)methyl iodide. The Sep-Pak C18 was conditioned by washing with acetonitrile, followed by water.

LCPAK00A1. The released glycans were separated from the peptides using a Sep-Pak C18 cartridge (Waters). Markush Group. The hydrophobic reversed phase material is retentive for most nonpolar compounds, and retains most organic analytes from aqueous matrices. Load the PNGaseF-digested sample onto the C18 column. Single-stranded C18 cartridge DNA purification ~0.5 nmol of DMT-on oligos can be cleaned up on ~2 mg of Sep-Pak C18. After reaction, the Sep Pak was rinsed with acetone to elute the labeled product, and the solvent evaporated rendering (11C)()methyl jasmonate at 96% radiochemical purity. VWR More>> Less<< VWRI470105-972 1 U The Sep-Pak C18 3 cc Vac Cartridge is a powerful tool that helps you attain your solid phase extraction goals. The solvent flow through the column was at a rate of 0.25 mL/min assisted by negative pressure at the column outlet generated by a vacuum manifold (Agilent .

Isot., 84 (2014), pp. At the end of each extraction run, after a cartridge filtering (Sep-Pak classic C18, Waters ), the extract was rotary evaporated (Laborota 4000, Heidolph Instruments GMBH and Co. KG, Schwabach, Germany) until all solvents were eliminated; the resulting dry extract was then dissolved in 100 g of ROO (Figure 1). Troubleshooting 39 Subsequent to clean-up using Sep-Pak cartridges as described above, standards and Suitable for HPLC, UHPLC, LC-MS and LC-MS/MS analyses. Markush Group. no.

2. . Acetone was removed in vacuo at 35?C and the aqueous residue purified using Sep-Pak C18 cartridges as described above.