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Solid Phase Extraction.

Sorbent Charge Can be Activated or Deactivated. These products are available in more than 200 chemistry and format combinations for convenient sample preparation methods based on reverse-phase, normal-phase, ion-exchange, and application-specific specialty chemistries.

Collect sample for analysis if desired compound has passed through the bed without being retained.

exchange/reversed phased) behavior provides greater flexibility in method design, the ability to fractionate neutral compounds, and cleaner extracts. This item has been discontinued by the manufacturer and is no longer available.

Filled with stationary phase compositions . Restek.

1. Solid-Phase Extraction (SPE) is a sample preparation technique that is used to clean up and/or concentrate samples before analysis. Details. PREVIOUS Glass Bottom Plates. Common in environmental Used for large scale purication Reverse SPE tubes prior to elution analysis to reduce leachables (medicinal chemistry purication) to minimize elution volume from PP hardware and PE frits for strongly retained compounds Supel-Tips SPE Rezorian Cartridges Discovery SPE 96-Well Plates Dispersive SPE

The hydrophobic reversed phase material is retentive for most nonpolar compounds, and retains most organic analytes from aqueous matrices. Use Thermo Scientific SOLA Solid Phase Extraction (SPE) cartridges and plates to help prevent costly reanalysis of bioanalytical samples by preventing blocking, voiding, and channeling during the SPE sample preparation process. Types.

Solid phase extraction (SPE) is a sample preparation technique often used by chromatographers prior to analysis. Award-winning fritless polymeric technology eliminates the issues encountered in conventional SPE so that your lab can:

Product Category. Particle Size: 5 m - 250 m. AttractSPE DVB is available under Disk and Powder format as well as under several SPE cartridges size from 1mL to 20mL , reversible formats and for automate. Reversed Phase Cartridges.

Resprep S-DVB SPE Cartridge. Thermo Scientific HyperSep C18 Cartridges feature a highly retentive alkyl-bonded phase for nonpolar to moderately polar compounds.

Copure C18N SPE Cartridges 2000mg/12mL 20/Box Write a Comment.

Reversed-phase SPE cartridges use non-polar adsorbents (carbohydrates or polymers) on the stationary phase to separate organic solutes from the polar phase (water).

. Steroids Extraction By Reversed-Phase Chromatography; SPE Extraction Of Oil & Grease From Water Using SiliaPrep C18 (17%) SPE Extraction Of Phenolic Compounds From Wine Using SiliaPrep C18 (17%) SPE Extraction Of Streptomycin From Food Using SiliaPrep SCX And C18 (17%) Reversed-phase SPE is considered the least selective retention mechanism when compared to normalphase or ion-exchange SPE.

AttractSPEHLB are uncharged water-wettable polymeric resin suitable for the extraction of a wide range of analytes (polar, apolar, neutral, acidic, basic). Chromatographers use solid phase extraction (SPE) as a sample preparation method-of-choice to improve analytical results by removing interfering compounds, extracting the analyte in a preferred solvent, and concentrating the analyte. A SPE cartridge consists of a small, open-ended container (often a syringe barrel) filled with particles coated with a sorbent. 5.

Thermo Scientific HyperSep solid phase extraction cartridges offer reproducible, reliable sample preparation in a traditional format. The bonded octadecyl functional group allows for increased binding capacities with higher recoveries. 50/Pk. Apply sample solution to the top of the packing bed.

SPE cartridges. Solid phase extraction (SPE) is a technique designed for rapid, selective sample preparation and purification prior to the chromatographic analysis (e.g.

Solid Phase Extraction Products SPE Tubes and Specialty Hardware Quick Look-Up Guide Additional Tubes and SPE Configurations Custom Capabilities Glass SPE Tubes with PTFE and SS Frits (pg.

22, 23 and 30) Reverse SPE tubes . 4-Step Method. Solid Phase Extraction . The analyte can be eluted by washing the cartridge with a non-polar solvent, which disrupts the interaction of the analyte and the stationary phase. Pore Size: 40 - 450 .

Description Reversed Phase C18A SPE Cartridges The C18A SPE cartridge uses octadecyl silica as a filler to extract non-polar compounds from an aqueous solution by hydrophobic interaction.

Solid phase extractions use the same type of stationary phases as are used in liquid chromatography columns.

Highly reproducible recovery rates; cartridge to cartridge, lot to lot. The type of SPE sorbent is chosen based on the chemical properties of the species we wish to separate. The C8 SPE cartridge is a moderately hydrophobic, reverse phase silica matrix filler that retains non-polar compounds through hydrophobic interactions. The present study reports a precise and simple offline solid-phase extraction (SPE) coupled with reversed-phase high-performance liquid chromatography (RP-HPLC) method for the simultaneous determination of five representative and commonly present pharmaceuticals and personal care products (PPCPs), a new class of emerging pollutants in the aquatic environment.

An important distinction to keep in mind when employing ion-exchange mechanisms is the nature of the charge state of the analyte. In other words, it may be difficult for a reversed-phase method or bonded chemistry to differentiate between molecules that are structurally similar. The solid phase is simply used to "filter" the sample.

In both cases, explosives can be eluted from the SPE cartridge already dissolved in reversed-phase HPLC-compatible solvents, greatly simplifying sample transfer.

Features of Chromabond and Chromafix SPE Portfolio. Compared with C18, C8 has shorter carbon chains and moderate hydrophobicity, thus makes an alternative for extracting compounds that is strongly retained by C18 sorbent.

Oasis HLB cartridges contain the Oasis HLB sorbent, which is a universal polymeric reversed phase sorbent that was developed for the extraction of a wide range of acidic, basic, and neutral compounds from various matrices using a simple, generic protocol.Chromatography columns separate molecules within mixtures during gas or liquid chromatography. Reversed phase SPE separates analytes based on their polarity.

The first step in using SPE is to select the correct type of adsorbent for the type of separation that is needed.

The precision and accuracy of the Thermo Scientific HyperSep C18 Cartridges Get improved retention for most nonpolar compounds and organic analytes in aqueous matrices with these hydrophobic reversed phase C18 cartridges.

The technique offers targeted selectivity for analytes of interest, versatility for a wide range of sample volumes, can be easily automated for high-throughput, and is performed by following quick and simple methods (condition, equilibrate, load, wash, elute).

Solid-phase extraction is designed for rapid sample preparation and purification prior to chromatographic analysis.

Perform solid phase extraction (SPE) using columns and 96-well plates for sample preparation that improves chromatographic results.

However, such blended SPE products do exist, allowing you to utilize ion-exchange and reversed-phase retention mechanisms in the same SPE medium (e.g., polymeric ion-exchange SPE sorbents). A novel 2-layer SPE cartridge efficiently reduces background for pesticide determination. 30 micron spherical (SFAR C18) 50 micron irregular (KP-C18-HS) Speciality Phase Cartridges.

The cartridge . Solid Phase Extraction (SPE) Cartridges & Well Plates . Reversed-Phase SPE; Normal-Phase SPE; Ion-Exchange SPE; Copure Adsorption SPE; Copure Mixed SPE; Copure Specialty SPE; Copure MS SPE; Copure SLE; Copure PPT; SPE Vacuum Manifolds; Life Science. Solid phase extraction (SPE) is a variant of liquid chromatography.

HLB SPE cartridges. 24050.

Solid Phase Extraction .

C18, SPE Cartridges, Oro-Sep, 300.0 mg, 100/pkg Reversed phase retention mechanism of the C18 elute allows for the extraction of hydrophobic compounds from aqueous matrices.

Reversed-phase SPE is considered the least selective retention mechanism when compared to normalphase or ion-exchange SPE.

C18 Solid Phase Extraction Spe Cartridge , Find Complete Details about C18 Solid Phase Extraction Spe Cartridge,Solid Phase Extraction,Spe Cartridge,200mg Spe Column from Supplier or Manufacturer-Hawach Scientific Co., Ltd. . HPLC, GC, TLC).

These correspond to the usual filling materials of HPLC columns, such as reversed phase, normal phase, ion exchange or mixed-mode phase. Female Luer fitting at the inlet, male Luer tip as exit. The C18 solid phase extraction (SPE) cartridge uses octadecyl silica as a filler to retain non-polar compounds by strong hydrophobic interaction. 24050. Ion Exchange. We mainly offer three basic packing types for normal phase, reverse phase and ion exchange extraction as follows: Silica-based C18, C18-ne, C8, C2, PHE, CN, PSA, NH2, SAX, SCX, Silica Polymer-based . Description. From Email Email notification Text Comment will be posted once reviewed.

External dry loading using reversed-phase media as the dry load sorbent is a very efficient way to protect your reversed-phase flash cartridge from contamination. 29 and 30) Common in environmental analysis to reduce leachables from PP hardware and PE frits Reversible SPE Tubes (pg. Copure C8 sorbent is composed of octyl-bounded silica gel particles and retains non-polar compounds by hydrophobic interactions.

C18 silica is end-capped. 3 mL, 500 mg. 500 mg/3 mL. Rinse packing Bed with 3-5 mL methanol.

It can retain most organic compounds and is widely used in areas such as environmental monitoring and food safety.

. Reversed-Phase SPE For extracting non-polar compounds from complex matrices, Biocomma offers several reversed-phase SPE cartridges: most commonly used C18 (endcapped and non-endcapped) and C8. SPE provides clean extracts and high recoveries.

Reversed-Phase SPE Sorbent surface is relatively hydrophobic -Sep-Pak C18 Silica, other alkyl silica, Oasis HLB Sample is prepared for SPE in aqueous or other polar sorbent Oasis HLB is better -pH stable from 1-14 -Doesn't de-wet Examples: Isolation and enrichment of organic contaminants from surface waters 3 mL, 500 mg. 500 mg/3 mL.

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Solid phase extraction (SPE) is an effective technique for cleaning up and concentrating samples, prior to HPLC or GC analysis.

Solid phase extractions (SPEs) are routinely used in the sample preparation for quantifying analytes in biological fluids such as plasma and urine. The hydrophobic reversed phase material is retentive for most nonpolar compounds, and retains most organic analytes from aqueous matrices. [SPE Column Silica gel-based Normal / Reverse Phase] .We manufacture and distribute chemical reagents for research use only or various antibodies.

Complementary Reversed Phase Retention of Neutral Compounds. .

Copure C18 sorbent is composed of endcapped octadecyl-bounded silica gel particles and retains non-polar compounds by hydrophobic interactions.

However, such blended SPE products do exist, allowing you to utilize ion-exchange and reversed-phase retention mechanisms in the same SPE medium (e.g., polymeric ion-exchange SPE sorbents). Compared with the C18 filler, the C8 filler has a shorter carbon chain and a weaker non-polar hydrophobic interaction. Typical applications include . The stationary phase of a reversed phase SPE cartridge retain compounds of low polarity due to the hydrophobic effect.

SPE cartridges are available with many different sorbents.

Excellent recovery rates, especially for the enrichment of pharmaceuticals and active ingredients, due to the spherical particle shape, homogeneous surface, and optimized pore structure.

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The analyte can be eluted by washing the cartridge with a non-polar solvent, which disrupts the interaction of the analyte and the stationary phase.

SPE is most often used to remove interfering compounds from a sample, although it can also be used to enrich/concentrate analytes of interest in the sample. Reversed phase (C18, C8, cyano, phenyl) Normal phase (silica, diol, NH 2) Ion .

28 Online SPE-HPLC was accomplished using a combination of two six-port valves.

Typical applications include . Applications using C18 silica SPE cartridges are found in the pharmaceutical, environmental, and forensic sci-ence industries. . Activation: Rinse the extraction cartridge with 3-5 ml methanol; rinse the extraction cartridge with 3-5 ml deionized water (or buffer) and keep the extraction cartridge moist 2.

The bonded octadecyl functional group allows for increased binding capacities with higher recoveries.

Solid phase extraction (SPE) is a much faster purification method if optimized and it can be done with the standard cartridges as well as 96-well plates, that allow many samples to efficiently be run at the same time. The force between non-polar substances is van der Waals attraction, and the affinity of the agent to the solute mainly depends on its hydrophobicity.

Wide range of over 25 adsorbents to meet any application need. Features of Reversed Phase C18 Solid Phase Extraction (SPE) Cartridges

HyperSep SPE Cartridges and Plates.

Rinse with 3 - 5 mL water or buffer. Product Code Product Name Filler Content (mg/cartridge) Package Size; 297-47451: Flush the cartridge with aqueous solvent to remove pH modifiers and buffers.

N/A. Push or draw through the bed at 1 - 5 mL/min. Excellent recovery rates, especially for the enrichment of pharmaceuticals and active ingredients, due to the spherical particle shape, homogeneous surface, and optimized pore structure. Restek.

Description. With reversed phase (RP) SPE, the dimethoxytrityl (DMT) group, that is attached to the

Reverse Phase. Activated carbon; 50 micron irregular (ISOLUTE-NH2)

. Catalog number: 60109-001. Product Overview Videos Documents Recommendations Showing 10 of 14 Show more

It is particularly suited for the extraction of weak acids (pKa 2 - 8).

This item has been discontinued by the manufacturer and is no longer available. Solid Phase Extraction (SPE) Application Notes.

Copure QuEChERS .

Reversed-Phase SPE; Copure C18N SPE Cartridges; . One clear advantage in SPE-HPLC of explosives, as demonstrated in this and the previous reference, is the . The hydrophobic reverse phase filler can retain most of the non-polar compounds and most of the organic analytes in the aqueous matrix. Reversed Phase SPE . Polymeric MCX SPE cartridges sorbent has a larger surface area compared with other sorbents, so it has a high ion exchange capacity which can keep alkaline material effectively Skip to content +86-29-89284429

Save Big with Phenomenex's Legendary Savings http://www.phenomenex.com/info/form/17yearendpromoA quick look at how Strata-X polymeric sorbents can simplify t.

This is the most commonly used sorbent for sample preparation prior HPLC or GC analysis.

N/A.

All Sep-Pak Cartridges contain the high quality sorbents and chemistries that provide highly reproducible SPE results.

Resprep S-DVB SPE Cartridge High-purity material with highest reproducibility and lowest blank values due to an optimized manufacturing process. C18, SPE Cartridges, Oro-Sep, 300.0 mg, 100/pkg Reversed phase retention mechanism of the C18 elute allows for the extraction of hydrophobic compounds from aqueous matrices.

2: Rinse packing bed with 3-5 mL water or buffer.

Always store reversed-phase cartridges in your method's strong solvent (without pH modifiers or .

The filler retains most of the organic matter and is widely used in the fields of environmental and food safety.

Remove unwanted contaminants including phospholipids, proteins, and particulates for superior chromatography Easily cleanup large or small volumes Strata-XL cleans up viscous samples as well as target analytes that exceed 10 kDa The 96-well plate microelution Strata-X formats are ideal for concentrated extraction for small volumes Order Now

The analyte is removed from the adsorbent by elution with a suitable solvent. However, because reversed-phase will retain most molecules with any . spe columnspe cartridgesolid phase extraction cartridgesolid phase extraction column. There are a variety of SPE stationary phases to choose from including: reverse phase, normal phase, ion-exchange, and mixed mode, with mixed mode being a mixture of both .

Resprep C18 SPE Cartridges. .

Using SiliaPrepX will guarantee the following:.

Suzuki Catalysts Scavenging Study with Activated Carbon and SiliaMetS; Amine sorbent for the separation of polar compounds; Using Silica-Based Products for Food and Drug Applications; Plastic Chemical Resistance Guide; Reversed-Phase Chromatography: General Introduction for Improved Method Development Reversed phase non-polar (hydrophobic) SPE .

Product Line: SOLA: Sorbent: Mixed Mode Weak Anion Exchanger, Polymeric: Type . Solid Phase Extraction.

Compared to liquid-liquid extraction, SPE is much faster, uses less solvent, and saves money. Solid-Phase Extraction (SPE) Columns and Cartridges . To perform reversed-phase chromatography with SPE cartridges, use a gradient of strongly to weakly polar solvents [from weak to strong solvent elution strength] with a non-polar packing material.

SOLA WAX SPE Cartridge: Bed Weight: 10 mg: For Use With (Application) Bioanalytical Applications - Strong Ion-Exchange Retention of Strong Acidic Compounds. The stationary phase of a reversed phase SPE cartridge is derivatized with hydrocarbon chains, which retain compounds of mid to low polarity due to the hydrophobic effect. Solid phase extraction (SPE) is a sample preparation technique often used by chromatographers prior to analysis. Copure C18A SPE Cartridges 100mg/1mL 100/Box COC18A3200 . AttractSPESAX is SPE based on strong anion exchange polymeric phase interacting with the analytes via a mixed mode retention mechanism: ion exchange with strong basic functional groups and reversed phase. Reversed Phase C18 SPE Cartridges Hawach Reversed Phase C18 SPE Cartridges have a high retention rate of the alkyl bonding phase for non-polar to moderate polar compounds. The following specifications of SPE cartridges are available: Volume: 0.01 ml - 200 ml.

The full range of solid phase extraction (SPE) well plates, columns, disks and accessories from Biotage. Chemistries. In other words, it may be difficult for a reversed-phase method or bonded chemistry to differentiate between molecules that are structurally similar.

High quality products covering a broad spectrum of applications for a wide range of complexe matrices. Thermo Scientific SOLA SPE Cartridges revolutionize solid phase extraction (SPE).

Available in silica and polymer-based phases for reverse phase, ion exchange, and specific-application needs.

HPLC, GC, TLC). QuEChERS AOAC Kits; QuEChERS EN Kits .

4-Step SPE Procedure. The Agilent SampliQ WAX solid-phase extraction cartridges are compatible with water, acid, basic solvents from pH 0 to 14, and most organic solvents.

Solid phase extraction (SPE) is a technique designed for rapid, selective sample preparation and purification prior to the chromatographic analysis (e.g. SPE phase selection overview. Please call customer service for assistance: 1-800-766-7000.

Some of these sorbents are the same types used in HPLC columns. .

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An important distinction to keep in mind when employing ion-exchange mechanisms is the nature of the charge state of the analyte.

Step 1: Conditioning.

This is the most commonly used sorbent for sample preparation prior HPLC or GC analysis.